Establishing the origin of azoospermia in patients with azoospermia and low volume ejaculates
Qualitative
Fructose
Semen
Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection.
Submit only 1 of the following specimens:
Specimen Type: Semen
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic container
Specimen Volume: Total ejaculate
Collection Instructions: Do not dilute specimen. Freeze specimen at -20 degrees C.
Specimen Type: Seminal plasma
Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. After semen collection, wait 30 to 40 minutes until the semen is liquefied, then centrifuge the semen for 10 minutes at maximum centrifuge speed.
2. Remove top 3/4 of specimen with a pipet and place in a plastic vial. Freeze specimen at -20 degrees C.
3. Discard remainder of centrifuged specimen.
0.5 mL
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Semen | Frozen |
Establishing the origin of azoospermia in patients with azoospermia and low volume ejaculates
Fructose is produced in the male reproductive tract by the seminal vesicles and is released into the semen during ejaculation. Fructose is the energy source for sperm motility.
Positive
A positive (indicated by color change) fructose is considered normal.
A semen specimen that contains no sperm (azoospermia) and is fructose negative may indicate an absence of the seminal vesicles, absence of the vas deferens in the area of the seminal vesicles, or an obstruction at the level of the seminal vesicles.
This test should be performed in conjunction with a semen analysis to determine semen volume, pH, sperm concentration, motility, and grade of forward progression.
Fructose test must be on a separate ejaculate.
1. Lipshultz LI, Howards SS, Niederberger CS, eds: In: Infertility in the Male. 4th ed. Cambridge University Press 2009
2. Risz B, Agarwal A, Sabanegh ES, eds: Male Infertility in Reproductive Medicine: Diagnosis and Management. CRC Press; 2019
A qualitative method using resorcinol will detect the presence or absence of fructose.(Keel BA, Webster BW: Handbook of the Laboratory Diagnosis and Treatment of Infertility. CRC Press: 1990:49)
Friday
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
82757
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
FROS2 | Qualitative Fructose,Semen | 13943-6 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
FRUCS | Fructose, Semen/Seminal P | 13943-6 |
CLSM | Collection Site | 56816-2 |
VOLSM | Semen Volume | 3160-9 |
CMT49 | Comment | 48767-8 |