Detecting Nocardia species and other partially-acid fast aerobic actinomycetes in clinical specimens
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
TISSR | Tissue Processing | No, (Bill Only) | No |
Modified Acid-Fast Stain
Fungal Smear
Nocardia Stain
Special Stain for Fungus, Nocardia
Aerobic Actinomycetes
Varies
Specimen source is required.
Question ID | Description | Answers |
---|---|---|
Q00M0056 | Specimen Source |
Container/Tube: Sterile container
Specimen Volume: 0.2 mL
Collection Instructions: Collect a raw specimen.
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
See Specimen Required
Blood Bone marrow Fixed tissue Viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) Environmental specimen Wood shaft or charcoal swab Prepared slides, glass slides, microscope slides | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Refrigerated (preferred) | 7 days | |
Ambient | 7 days |
Detecting Nocardia species and other partially-acid fast aerobic actinomycetes in clinical specimens
Nocardia species and other aerobic actinomycetes can cause significant disease in immunocompromised patients. Clinical presentations can include, but are not limited to, pneumonia, skin abscess, bacteremia, brain abscess, eye infection, and joint infection.
The modified acid-fast stain can detect Nocardia species and other partially-acid fast aerobic actinomycetes directly from clinical specimens.
Reported as positive or negative
Patients whose specimens are reported as partially acid-fast positive should be considered potentially infected with Nocardia species or other aerobic actinomycetes, pending definitive diagnosis by molecular methods or culture.
Artifacts may exhibit nonspecific staining and be confused with organisms. Not all aerobic actinomycetes stain as partially acid-fast. A negative stain does not rule out the possibility of infection and culture should be performed. Nocardia species and other aerobic actinomycetes grow well on mycobacterial culture medium so CTB / Mycobacteria and Nocardia Culture, Varies should be ordered. Sometimes, Nocardia species can also be recovered in fungal cultures (FGEN / Fungal Culture, Routine).
Brown-Elliott BA, Brown JM, Conville PS, Wallace RJ. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin Microbiol Rev. 2006;19(2):259-282
Modified acid-fast stain. This method uses carbolfuchsin stain (Kinyoun method) with a milder decolorization step for detection of Nocardia species.(Atlas R, Snyder J. Reagents, stains and media: Bacteriology. In: Carrol KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology, 12th ed. ASM Press; 2019:331-361)
Monday through Sunday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
87206
87176-Tissue processing (if appropriate)
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
SSF1 | Nocardia Stain | 21003-9 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
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SSF1 | Nocardia Stain | In Process |