Test Catalog

Test Id : ANIDE

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Organism Referred for Identification, Anaerobic Bacteria

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Identifying anaerobic bacteria involved in human infections

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
RMALA Id MALDI-TOF Mass Spec Anaerobe No, (Bill Only) No
ANAID Anaerobe Ident No, (Bill Only) No
ISAN Anaerobe Ident by Sequencing No, (Bill Only) No
COMM Identification Commercial Kit No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
STAP Identification Staphylococcus No, (Bill Only) No
STRP Identification Streptococcus No, (Bill Only) No
SIDC Ident Serologic Agglut Method 4 No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will be identified and billed as appropriate.

Method Name
A short description of the method used to perform the test

Dependent on organism submitted, 1 or more of the following methods will be used: Media, Aero Tolerance Testing, Conventional Biochemical Tests, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry, or 16S RNA Gene Sequencing

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Organism Ref for ID, Anaerobic Bact

Aliases
Lists additional common names for a test, as an aid in searching

Anaerobic

Anaerobic identification

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will be identified and billed as appropriate.

Specimen Type
Describes the specimen type validated for testing

Varies

Ordering Guidance

If susceptibility testing is needed; order MMLSA / Antimicrobial Susceptibility, Anaerobic Bacteria, Minimal Inhibitory Concentration, Varies also. If susceptibilities are not appropriate, MMLSA will be canceled at report time.

Shipping Instructions

1. For shipping information see Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.

Necessary Information

1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
Q00M0004 Specimen Source (Required) and Isolate Description-Gram reaction, morphology, tests performed (Required)

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Specimen Type: Pure culture of organism from a source not normally colonized by anaerobes

Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds

Supplies:

-Anaerobic Transport Tube (T588)

-Infectious Container, Large (T146)

Container/Tube:

Preferred: Anaerobic transport tube

Acceptable: Thioglycollate broth or any other suitable anaerobic transport system

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

See Specimen Required

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Agar plate
Received frozen 		Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
Refrigerated

Useful For
Suggests clinical disorders or settings where the test may be helpful

Identifying anaerobic bacteria involved in human infections

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will be identified and billed as appropriate.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Anaerobic bacteria are the greatest component of the human body's normal bacterial microbiota colonizing the skin, oral cavity, and genitourinary and lower gastrointestinal tracts. Their presence is important in promoting vitamin and other nutrient absorption and in preventing infection with disease-causing bacteria.

 

Anaerobes generally are of low pathogenicity but may possess virulence factors, such as endotoxin or polysaccharide capsules, or produce extracellular toxins. Disease occurs when a large inoculum develops in an area lacking oxygen or with a poor blood supply.

 

Typical anaerobic infections include peritonitis, abdominal or pelvic abscesses, endometritis, pelvic inflammatory disease, aspiration pneumonia, empyema, lung abscesses, sinusitis, brain abscesses, gas gangrene, and other soft tissue infections. Many Bacteroides produce beta-lactamase and are resistant to penicillins and cephalosporins. Imipenem, metronidazole, and clindamycin are effective agents, although resistance to clindamycin is increasing.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Identification of organism

Interpretation
Provides information to assist in interpretation of the test results

Isolation of anaerobes in significant numbers from well-collected specimens from blood, other normally sterile body fluids, or closed collections of purulent fluid indicates infection with the identified organism.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Jousimies-Somer HR, Summanen P, Citron DM, et al: Wadsworth Anaerobic Bacteriology Manual. 6th ed. Star Publishing Co; 2002

2. Baron EJ: Approaches to identification of anaerobic bacteria. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:905-908

3. Hall GS: Anaerobic bacteriology. In: Garcia LS, ed. Clinical Microbiology Procedures Handbook. Vol 1. 3rd ed. ASM Press; 2010:section 4

4. Song Y, Finegold SM: Peptostreptococcus, Finegoldia, Anaerococcus, Peptoniphilus, Veillonella, and other anaerobic cocci. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:909-919

5. Hall V, Copsey SD: Propionibacterium, Lactobacillus, Actinomyces, and other non-spore-forming anaerobic gram-positive rods. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:920-939

6. Stevens DL, Bryant AE, Carroll K: Clostridium. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:940-966

7. Kononen E, Conrads G, Nagy E: Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic gram-negative rods. In: Jorgensen JH, Carroll KC. Funke G, et al, eds. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:967-993

Method Description
Describes how the test is performed and provides a method-specific reference

Appropriately transported organisms are inoculated onto blood agar plates and into thioglycollate broth tubes. After 48 hours of incubation at 35 degrees C in an anaerobic atmosphere, colonies are identified using one or a combination of the following techniques: Gram stain, use of various differential media, aero tolerance testing, conventional biochemical tests, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, or 16S ribosomal RNA gene sequencing.(Procop, GW, Church DL, Hall GS, et al: The anaerobic bacteria. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott, Williams and Wilkins; 2017:chap 16)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

8 to 14 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

30 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87076-Organism ref for ID, anaerobic bact

87076-Id MALDI-TOF mass spec anaerobe (if appropriate)

87076-Anaerobe Ident (if appropriate)

87153-Anaerobe ident by sequencing (if appropriate)

87077-Identification commercial kit (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria identification (if appropriate)

87077-Additional identification procedure (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87147 x 3-Serologic agglut method 1 ident (if appropriate)

87147-Serologic agglut method 2 ident (if appropriate)

87147 x 4-Serologic agglut method 3 ident (if appropriate)

87147 x 2-6-Serologic Agglut Method 4 Ident (if appropriate)

87153-Aerobe ident by sequencing (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
ANIDE Organism Ref for ID, Anaerobic Bact 20878-5
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
ANIDE Organism Ref for ID, Anaerobic Bact 20878-5

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports