Test Catalog

Test Id : F8IS

Not Orderable

Coagulation Factor VIII Inhibitor Screen, Plasma

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Detecting the presence of a specific factor inhibitor directed against coagulation factor VIII

Method Name
A short description of the method used to perform the test

Only orderable as a reflex. For more information see:

8INHE / Factor VIII Inhibitor Evaluation, Plasma

ALUPP / Lupus Anticoagulant Profile, Plasma

ALBLD / Bleeding Diathesis Profile, Limited, Plasma

APROL / Prolonged Clot Time Profile, Plasma

AVWPR / von Willebrand Disease Profile, Plasma

 

Optical Clot-Based

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Coag Factor VIII Assay Inhib Scrn,P

Specimen Type
Describes the specimen type validated for testing

Plasma Na Cit

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Only orderable as a reflex. For more information see:

8INHE / Factor VIII Inhibitor Evaluation, Plasma

ALUPP / Lupus Anticoagulant Profile, Plasma

ALBLD / Bleeding Diathesis Profile, Limited, Plasma

APROL / Prolonged Clot Time Profile, Plasma

AVWPR / von Willebrand Disease Profile, Plasma

 

For more information see Coagulation Guidelines for Specimen Handling and Processing.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

2 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Detecting the presence of a specific factor inhibitor directed against coagulation factor VIII

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Specific factor inhibitors are antibodies that are found most often in response to the use of factor VIII concentrate by patients congenitally deficient in factor VIII (hemophilia A). Factor VIII inhibitors can also develop in non-hemophiliac patients (not previously factor VIII deficient), most commonly in the following: the elderly, postpartum patients, and patients with autoimmune disease. Testing will include coagulation factor VIII activity assay with dilutions to evaluate assay inhibition, and if the factor VIII assay activity is decreased, an inhibitor screen to look for specific factor VIII inhibition. If specific inhibition is apparent, it will be titered.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Only orderable as a reflex. For more information see:

8INHE / Factor VIII Inhibitor Evaluation, Plasma

ALUPP / Lupus Anticoagulant Profile, Plasma

ALBLD / Bleeding Diathesis Profile, Limited, Plasma

APROL / Prolonged Clot Time Profile, Plasma

AVWPR / von Willebrand Disease Profile, Plasma

 

Negative

Interpretation
Provides information to assist in interpretation of the test results

When testing is complete, if factor activity results fall within clinically normal ranges, an interpretive comment will be provided noting that inhibitor testing was not indicated and, therefore, not performed. If factor activity indicates the performance of inhibitor screen testing, an interpretive comment will be provided noting the presence or absence of a factor VIII inhibitor.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Occasionally, a potent lupus-like anticoagulant may cause false-positive results for a specific factor inhibitor (eg, factor VIII or IX).

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Bowie EJW, Thompson JH Jr, Didisheim P, Owen CA Jr. Mayo Clinic Laboratory Manual of Hemostasis. WB Saunders Company; 1971:111-115

2. Kasper CK. Treatment of factor VIII inhibitors. Prog Hemost Thromb. 1989;9:57-86

3. Peerschke EI, Castellone DD, Ledford-Kraemer M, et al. Laboratory assessment of FVIII inhibitor titer. Am J Clin Pathol. 2009;131(4):552-558

4. Pruthi RK, Nichols WL. Autoimmune factor VIII inhibitors. Curr Opin Hematol. 1999;6(5):314-322

5. Laboratory Hematology Practice. In: Kottke-Marchant K, ed. Wiley Blackwell Publishing; 2012

6. Hematology: Basic Principles and Practice. In: Hoffman R, Benz EJ Jr, Silberstein LE, ed. 7th ed. Elsevier; 2018

Method Description
Describes how the test is performed and provides a method-specific reference

The factor VIII inhibitor screen is performed on the Instrumentation Laboratory ACL TOP. The assay consists of measuring the factor VIII activity (activated partial thromboplastin time based assay) before and after incubation of a mixture of normal plasma and patient's plasma for 1 hour at 37 degrees C. Interpretation of the presence or absence of the indication of a factor VIII inhibitor is determined by comparing the factor VIII activity results and the calculated expected values.(Owen CA Jr, Bowie EJW, Thompson JH Jr. The Diagnosis of Bleeding Disorders. 7nd ed. Little Brown and Company; 1975:143-145; Meijer P, Verbruggen HW, Spannagi M. Chapter 33: Clotting factors and inhibitors: Assays and Interpretation. In: Kottke-Marchant K, ed. Laboratory Hematology Practice. Wiley Blackwell Publishing; 2012:435-446)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 3 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

85335

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
F8IS Coag Factor VIII Assay Inhib Scrn,P 3206-0
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
7289 Coag Factor VIII Assay Inhib Scrn,P 3206-0

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports