Determining whether Aeromonas species may be the cause of diarrhea
This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.
This test provides evidence of the presence of the bacterium, Aeromonas species, in feces, in a viable state, and provides an isolate for antibacterial susceptibility testing.
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
GID | Bacteria Identification | No, (Bill Only) | No |
ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
REFID | Additional Identification Procedure | No, (Bill Only) | No |
RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
When this test is ordered, the reflex tests may be performed and charged separately.
For more information see Laboratory Testing for Infectious Causes of Diarrhea.
Conventional Culture
Aerobic Bacterial Stool Culture
Enteric pathogens
Aeromonas Stool Culture
Aeromonas
Stool Culture
When this test is ordered, the reflex tests may be performed and charged separately.
For more information see Laboratory Testing for Infectious Causes of Diarrhea.
Fecal
If susceptibility testing is needed, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Aeromonas species from feces), it will not be performed and the ZMMLS order will be canceled at time of report.
In some cases, local public health requirements may impact Mayo Clinic Laboratories clients, requiring, for example, submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Aeromonas species recovered from specimens submitted to Mayo Clinic Laboratories by calling 800-533-1710 as soon as possible after reporting (to ensure viability of the bacterium).
Specimen must arrive within 96 hours of collection.
Specimen source is required.
Question ID | Description | Answers |
---|---|---|
Q00M0081 | Specimen Source |
Patient Preparation: Medications: Do not use barium or bismuth before collection of specimen.
Supplies: Culture and Sensitivity Stool Transport Vial (T058)
Specimen Type: Preserved Feces
Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)
Specimen Volume: Representative portion of fecal specimen
Collection Instructions:
1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
3. Place vial in a sealed plastic bag.
1 mL
ECOFIX preservative; formalin, or PVA fixative | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Fecal | Ambient (preferred) | 4 days | |
Refrigerated | 4 days |
Determining whether Aeromonas species may be the cause of diarrhea
This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.
When this test is ordered, the reflex tests may be performed and charged separately.
For more information see Laboratory Testing for Infectious Causes of Diarrhea.
Diarrhea may be caused by a number of agents, including bacteria, viruses, parasites, and chemicals; these agents may result in similar symptoms. A thorough patient history covering symptoms, severity and duration of illness, age, travel history, food consumption, history of recent antibiotic use, and illnesses in the family or other contacts will help the healthcare provider determine the appropriate testing to be performed.
Aeromonas species are associated with a range of diarrheal presentations including acute secretory diarrhea with vomiting, chronic diarrhea lasting more than 10 days, and traveler’s diarrhea. Most cases of Aeromonas-associated diarrhea are self-limited and may be managed with supportive therapy (eg, rehydration), but in severe cases or those in patients with a history of immunosuppression, antimicrobial therapy may be considered. Clinical studies have demonstrated differences in antimicrobial susceptibility profiles between Aeromonas species, highlighting the importance of both species identification and susceptibility testing for all isolates, particularly in serious infections.
No growth of pathogen
The growth of Aeromonas species identifies a potential cause of diarrhea.
The yield of Aeromonas species is reduced when specimens are delayed in transit to the laboratory (>2 hours from collection for unpreserved specimens).
Check local public health requirements, which may require submission of isolates to a public health laboratory.
1. Pillai DR. Fecal culture for aerobic pathogens of gastroenteritis. In: Clinical Microbiology Procedures Handbook, 4th ed. ASM Press; 2016, Section 3.8.1
2. Pillai DR. Fecal culture for Campylobacter and related organisms. In: Clinical Microbiology Procedures Handbook, 4th ed. ASM Press; 2016, Section 3.8.2
3. DuPont HL. Persistent diarrhea: A clinical review. JAMA. 2016;315(24):2712-2723 doi:10.1001/jama.2016.7833
The fecal specimen is inoculated onto sheep blood agar and cefsulodin-irgasan-novobiocin (CIN) agar. After incubation, suspect colonies are identified using one or a combination of the following techniques: matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, serologic methods, or nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene. Isolates are reported as Aeromonas caviae complex; Aeromonas hydrophila complex; or Aeromonas veronii complex.(Lamy B, Horneman AJ. Aeromonas. In: Jorgensen J, Pfaller M, et al. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:765-774)
Monday through Sunday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
87046-Aeromonas Culture, Feces-with isolation and preliminary examination
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
AERMC | Aeromonas Culture, F | 30122-6 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
AERMC | Aeromonas Culture, F | 30122-6 |