Test Catalog

Test Id : LPSBF

Order This Test

Lipase, Body Fluid

Download
Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining whether pancreatic inflammation or pancreatic fistula may be contributing to a pathological accumulation of fluid

Method Name
A short description of the method used to perform the test

Enzymatic Colorimetric Reaction

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

No

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Lipase, BF

Specimen Type
Describes the specimen type validated for testing

Body Fluid

Necessary Information

1. Date and time of collection are required.

2. Specimen source is required.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
FLD7 Fluid Type, Lipase

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Specimen Type: Body fluid

Preferred Sources:

-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)

-Pleural fluid (pleural, chest, thoracentesis)

-Drain fluid (drainage, Jackson Pratt [JP] drain)

-Pericardial

Acceptable Source: Write in source name with source location (if appropriate)

Collection Container/Tube: Sterile container

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge to remove any cellular material and transfer into a plastic vial.

2. Indicate the specimen source and source location on label.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

0.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Cerebrospinal fluid, feces, breast milk, saliva, nasal secretions, gastric secretions, bronchoalveolar lavage (BAL) or bronchial washings, sputum, synovial, colostomy/ostomy, urine, or vitreous fluid Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Body Fluid Refrigerated (preferred) 7 days
Frozen 30 days
Ambient 24 hours

Useful For
Suggests clinical disorders or settings where the test may be helpful

Determining whether pancreatic inflammation or pancreatic fistula may be contributing to a pathological accumulation of fluid

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Lipases are enzymes that hydrolyze glycerol esters of long-chain fatty acids and produce fatty acids and 2-acylglycerol. The pancreas is the primary source of serum lipase. Pancreatic injury results in increased serum lipase levels. Serum lipase is measured to aid in the diagnosis of pancreatitis.

 

Peritoneal fluid:

The digestive enzymes amylase and lipase can be measured in the identification of pancreatic fluid in the peritoneal cavity. Concentrations are expected to be elevated and at least several-fold times higher in fluid of pancreatic origin compared to simultaneous concentrations in serum.(1,2)

 

Drain fluid:

Lipase is expected to be elevated in drain fluids formed due to chronic pancreatitis or formation of a fistula following surgery.(1,3,4) Comparison to serum concentrations is recommended with elevations several-fold higher than blood being suggestive of the presence of pancreatic fluid in the drained cavity.(5)

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

An interpretive report will be provided.

Interpretation
Provides information to assist in interpretation of the test results

Fluids (peritoneal, drain):

Lipase concentrations several-fold higher than serum lipase concentrations is suggestive of the presence of pancreatic fluid in the drained cavity.

 

All other fluids:

Body fluid lipase activity may become elevated due to the presence of pancreatic fluid in the drained cavity. Results should be interpreted in conjunction with serum lipase and other clinical findings.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

In very rare cases of gammopathy, in particular type IgM (Waldenstrom macroglobulinemia) may cause unreliable results.

 

Calcium dobesilate causes artificially low lipase results.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Block DR, Florkowski CM. Body Fluids. In: Rifai N, Horvath AR, Wittwer CT. eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier;2018:chap 43

2. Robert JH, Meyer P, Rohner A. Can serum and peritoneal amylase and lipase determinations help in the early prognosis of acute pancreatitis? Ann Surg. 1986;203(2):163-168. doi:10.1097/00000658-198602000-00009

3. Lipsett PA, Cameron JL. Internal pancreatic fistula. Am J Surg. 1992;163(2):216-220. doi:10.1016/0002-9610(92)90104-y

4. Kaman L, Behera A, Singh R, Katariya RN. Internal pancreatic fistulas with pancreatic ascites and pancreatic pleural effusions: recognition and management. ANZ J Surg. 200;71(4):221-225. doi:10.1046/j.1440-1622.2001.02077.x

5. Sileo AV, Chawla SK, LoPresti PA: Pancreatic ascites: Diagnostic importance of ascitic lipase. Am J Dig Dis. 1975 Dec;20(12):1110-1114. doi:10.1007/BF01070753

6. Nandakumar V, Dolan C, Baumann NA, et al. Effect of pH on the quantification of body fluid analytes for clinical diagnostic testing. Am J Clin Path. 2019; 152(1):S10-S11

Method Description
Describes how the test is performed and provides a method-specific reference

This is an enzymatic colorimetric assay with 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methyl-resorufin) ester as substrate. The chromogenic lipase substrate 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester is cleaved by the catalytic action of alkaline lipase solution to form 1,2-O-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6-methylresorufin) ester. This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. Addition of detergent and colipase increases the specificity of the assay for pancreatic lipase.

 

The color intensity of the red dye formed is directly proportional to the lipase activity and can be determined photometrically.(Package insert: LIPC, Lipase Colorimetric Assay. Roche Diagnostics; v12.0, 11/2015)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Jacksonville

Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

83690

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
LPSBF Lipase, BF 15212-4
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
LPBF Lipase, BF 15212-4
FLD7 Fluid Type, Lipase 14725-6

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports