Test Catalog

Test Id : CYTH1

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T-Helper Cell Type 1 Cytokine Panel, Plasma

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Assessment of plasma cytokine levels to understand the etiology of autoimmune and proinflammatory conditions when used in conjunction with clinical information and other laboratory testing

Method Name
A short description of the method used to perform the test

Bead-Based Multiplex Immunoassay

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Th1 Cytokine Panel, P

Aliases
Lists additional common names for a test, as an aid in searching

TNFa

IFNg

IL-2

sIL-2Ra

IL-12p70

Tumor Necrosis Factor - alpha

Interferon gamma

Interleukin 2

Interleukin 2 Receptor, Soluble

Interleukin 12

Specimen Type
Describes the specimen type validated for testing

Plasma EDTA

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Within 2 hours of collection, centrifuge at 4 degrees C, 1500 x g for 10 minutes and aliquot plasma into a plastic vial

3. Freeze immediately.

Additional Information:

1. Plasma for this test cannot be shared with other tests. Submit specimen for this test in its own vial.

2. This test cannot be added on to a previously collected specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

0.3 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-treated specimen Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen 21 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Assessment of plasma cytokine levels to understand the etiology of autoimmune and proinflammatory conditions when used in conjunction with clinical information and other laboratory testing

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Cytokines are important mediators of cell-to-cell communication within the innate and adaptive immune systems.  The expression of most cytokines is highly regulated, and generally occurs in response to foreign or self-antigenic stimulation. The functions of cytokines are extremely varied, with many cytokines also displaying pleiotropic effects, depending on their cellular target. As a group, cytokines and their receptors represent a highly complex and critical regulator of a normal immune response.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

TNF:

<18 years: Not established

> or =18 years: <10.0 pg/mL

 

IFN-gamma:

<18 years: Not established

> or =18 years: <60.0 pg/mL

 

IL-2:

<18 years: Not established

> or =18 years: <60.0 pg/mL

 

IL-2 receptor alpha soluble:

<18 years: Not established

> or =18 years: < or =959 pg/mL

 

IL-12p70:

<18 years: Not established

> or =18 years: <200 pg/mL

Interpretation
Provides information to assist in interpretation of the test results

Elevated cytokine concentrations could be consistent with the presence of infection or other inflammatory process.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Results from cytokine testing should not be used to establish or exclude a specific diagnosis.

 

Cytokine testing should only be used in conjunction with clinical information and other laboratory testing as part of a patient's overall assessment.

 

Normal concentrations of cytokines do not exclude the possibility of infection or other inflammatory condition.

 

Cytokine concentrations could be affected by immunomodulatory agents.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Cerrillo E, Moret I, Iborra M, et al. A nomogram combining fecal calprotectin levels and plasma cytokine profiles for individual prediction of postoperative Crohn's disease recurrence. Inflamm Bowel Dis. 2019;25(10):1681-1691. doi:10.1093/ibd/izz053

2. Li S, Hao X, Gong Y, et al. Effect of shenling baizhu powder on the serum TH1 cytokines of elderly patients with ulcerative colitis complicated by bloody purulent stool. Am J Transl Res. 2021;13(8):9701-9707

3. Abdelrahman AH, Salama, II, Salama SI, et al. Role of some serum biomarkers in the early detection of diabetic cardiomyopathy. Future Sci OA. 2021;7(5):FSO682. doi:10.2144/fsoa-2020-0184

4. Weng L, Chen Y, Liang T, et al. Biomarkers of interstitial lung disease associated with primary Sjogren's syndrome. Eur J Med Res. 2022;27(1):199. doi:10.1186/s40001-022-00828-3

5. Popova V, Geneva-Popova M, Kraev K, Batalov A. Assessment of TNF-alpha expression in unstable atherosclerotic plaques, serum IL-6 and TNF-alpha levels in patients with acute coronary syndrome and rheumatoid arthritis. Rheumatol Int. 2022;42(9):1589-1596. doi:10.1007/s00296-022-05113-4

6. Li L, Chen B, Zhao H, Wang G. Bone changes and curative effect of infliximab in patients with ankylosing spondylitis. J Musculoskelet Neuronal Interact. 2020;20(3):437-443

7. Zhao S, Liu Y, Zhou C, et al. Prediction model of delayed graft function based on clinical characteristics combined with serum IL-2 levels. BMC Nephrol. 2022;23(1):284. doi:10.1186/s12882-022-02908-2

8. Ye Q, Shao WX, Xu XJ, Yang YZ. The clinical application value of cytokines in treating infectious diseases. PLoS One. 2014;9(6):e98745. doi:10.1371/journal.pone.0098745

9. Wang Q, Wang C, Yang M, Li X, Cui J, Wang C. Diagnostic efficacy of serum cytokines and chemokines in patients with candidemia and bacteremia. Cytokine. 2020;130:155081. doi:10.1016/j.cyto.2020.155081

Method Description
Describes how the test is performed and provides a method-specific reference

Testing for plasma cytokine levels is accomplished using a laboratory-developed immunoassay.(Unpublished Mayo method)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Thursday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

2 to 8 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

83520 x5

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
CYTH1 Th1 Cytokine Panel, P 82335-1
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
622037 TNF 3074-2
622038 IFN-gamma 27415-9
622039 IL-2 33939-0
622040 IL-2 receptor alpha soluble 76039-7
622041 IL-12p70 41760-0

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports

Test Update Resources

Change Type Effective Date
New Test 2024-11-07