Test Id : MCGYN

When this test is ordered, slide review will always be performed at an additional charge.

Multiple oncology (cancer) gene panels are available. For more information see Hematology, Oncology, and Hereditary Test Selection Guide.

A pathology report (final or preliminary), at minimum containing the following information, must accompany specimen for testing to be performed:

1. Patient name

2. Block number-must be on all blocks, slides, and paperwork (can be handwritten on the paperwork)

3. Tissue collection date

4. Source of the tissue

This assay requires at least 20% tumor nuclei.

-Preferred amount of tumor area with sufficient percent tumor nuclei: tissue 216 mm(2)

-Minimum amount of tumor area: tissue 36 mm(2)

-These amounts are cumulative over up to 10 unstained slides and must have adequate percent tumor nuclei.

-Tissue fixation: 10% neutral buffered formalin, not decalcified

-For specimen preparation guidance, see Tissue Requirement for Solid Tumor Next-Generation Sequencing. In this document, the sizes are given as 4 mm x 4 mm x 10 slides as preferred: approximate/equivalent to 144 mm(2) and the minimum as 3 mm x 1 mm x 10 slides: approximate/equivalent to 36 mm(2).

Preferred:

Specimen Type: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue.

Acceptable:

Specimen Type: Tissue slide

Slides: 1 Stained and 10 unstained

Collection Instructions: Submit 1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides with 5-micron thick sections of the tumor tissue.

Note: The total amount of required tumor nuclei can be obtained by scraping up to 10 slides from the same block

Additional Information: Unused unstained slides will not be returned.

Specimen Type: Cytology slide (direct smears or ThinPrep)

Slides: 1 to 3 Slides

Collection Instructions: Submit 1 to 3 slides stained and coverslipped with a preferred total of 5000 nucleated cells or a minimum of at least 3000 nucleated cells.

Note: Glass coverslips are preferred; plastic coverslips are acceptable but will result in longer turnaround times.

Additional Information: Cytology slides will not be returned.

• Tissue Requirements for Solid Tumor Next-Generation Sequencing
• Targeted Genes and Methodology Details for MayoComplete Gynecological Panel

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.

See Specimen Required

Predicting patients' prognosis and response to targeted therapy

Assessment of microsatellite instability for immunotherapy decisions

This test uses targeted next-generation sequencing to determine microsatellite instability (MSI) status and evaluate for somatic mutations within the AKT1, APC, ARID1A, ARID1B, ATM, ATR, ATRX, BAP1, BARD1, BRAF, BRCA1, BRCA2, BRIP1, CDC42, CDK12, CDKN2A, CTNNB1, DICER1, EIF1AX, EPCAM, ERBB2, FBXW7, FOXL2, GNAS, KRAS, L1CAM, MED12, MLH1, MSH2, MSH6, NBN, NF2, NRAS, PALB2, PIK3CA, PIK3R1, PMS2, POLE, PPP2R1A, PTEN, RAD51C, RAD51D, RB1, SMARCA2, SMARCA4, SMARCB1, STK11, TP53, and TRAF7 genes. See Targeted Genes and Methodology Details for MayoComplete Gynecological Panel for details regarding the targeted gene regions evaluated by this test.

This test is performed to evaluate for somatic mutations within solid tumor samples. This test does not assess for germline alterations within the genes listed.

When this test is ordered, slide review will always be performed at an additional charge.

Molecular genetic profiling identifies targets amenable to targeted therapies, minimizing treatment costs and therapy-associated risks. Microsatellite instability (MSI) status is an increasingly important biomarker for determining effective immunotherapeutic treatment options for patients with solid tumors.

This test uses formalin-fixed paraffin-embedded tissue or cytology slides to assess for somatic mutations in the following genes known to be associated with gynecologic cancer: AKT1, APC, ARID1A, ARID1B, ATM, ATR, ATRX, BAP1, BARD1, BRAF, BRCA1, BRCA2, BRIP1, CDC42, CDK12, CDKN2A, CTNNB1, DICER1, EIF1AX, EPCAM, ERBB2, FBXW7, FOXL2, GNAS, KRAS, L1CAM, MED12, MLH1, MSH2, MSH6, NBN, NF2, NRAS, PALB2, PIK3CA, PIK3R1, PMS2, POLE, PPP2R1A, PTEN, RAD51C, RAD51D, RB1, SMARCA2, SMARCA4, SMARCB1, STK11, TP53, and TRAF7, as well as MSI status. The results of this test can be useful for molecular classification, assessing prognosis, and guiding treatment of individuals with gynecologic tumors, including endometrial, ovarian, and sex-cord stromal tumors. The data can also be used to help determine clinical trial eligibility.

An interpretive report will be provided.

The interpretation of molecular biomarker analysis includes an overview of the results and the associated diagnostic, prognostic, and therapeutic implications.

This test cannot differentiate between somatic and germline alterations. Additional testing may be necessary to clarify the significance of results if there is a potential hereditary risk.

DNA variants of uncertain significance may be identified.

A negative result does not rule out the presence of a variant that may be present below the limits of detection of this assay. In a specimen with 20% or more tumor content, the analytical sensitivity of this assay for sequence reportable alterations is 5% mutant allele frequency with a minimum coverage of 500X.

Point mutations and small deletion-insertion mutations will be detected in the AKT1, APC, ARID1A, ARID1B, ATM, ATR, ATRX, BAP1, BARD1, BRAF, BRCA1, BRCA2, BRIP1, CDK12, CDC42, CDKN2A, CTNNB1, DICER1, EPCAM, EIF1AX, ERBB2, FBXW7, FOXL2, GNAS, KRAS, L1CAM, MED12, MLH1, MSH2, MSH6, NBN, NF2, NRAS, PALB2, PIK3CA, PIK3R1, PMS2, POLE, PPP2R1A, PTEN, RAD51C, RAD51D, RB1, SMARCA2, SMARCA4, SMARCB1, STK11, TP53, and TRAF7 genes only. This test may detect single exon deletions but does not detect multi-exon deletions, duplications, or genomic copy number variants.

Variant allele frequency (VAF) is the percentage of sequencing reads supporting a specific variant divided by the total sequencing reads at that position. In somatic testing, VAF should be interpreted in the context of several factors, including, but not limited to, tumor purity/heterogeneity/copy number status (ploidy, gains/losses, loss of heterozygosity) and sequencing artifact/misalignment.(2,3)

Rare alterations (ie, polymorphisms) may be present that could lead to false-negative or false-positive results.

The presence or absence of a variant may not be predictive of response to therapy in all patients.

Test results should be interpreted in the context of clinical, tumor sampling, histopathological, and other laboratory data. If results obtained do not match other clinical or laboratory findings, contact the laboratory for discussion. Misinterpretation of results may occur if the information provided is inaccurate or incomplete.

Reliable results are dependent on adequate specimen collection and processing. This test has been validated on cytology slides and formalin-fixed, paraffin-embedded tissues; other types of fixatives are discouraged. Improper treatment of tissues, such as decalcification, may cause polymerase chain reaction failure.

Performance Characteristics:

The limit of detection for calling a somatic variant (single nucleotide variants [SNV] and deletions-insertions [delins, formerly indel]) is 5% variant allele frequency (VAF) and having at least 500x deduplicated coverage.

Verification studies demonstrated concordance between this test and the reference method for detection of SNV and delins is 98.5% (673/683) and 98.4% (122/124) of variants, respectively. Concordance for the detection of delins was 99.0% (100/101) in variants 1 to 10 base pairs (bp) in size, 93.3% (14/15) in variants 11 to 50 bp in size, and 100% (8/8) in variants over 50 bp in size.

Microsatellite instability (MSI) evaluation is accurate at a tumor purity of at least 10% for colorectal tumors and 20% for other tumor types. During verification studies, 98% (200/204) concordance for MSI status was observed between this test and the reference method.

To ensure accuracy, this test will be performed on cases that are estimated by a pathologist to have at least 20% tumor cells.

1. WHO Classification of Tumours Editorial Board, eds. Female genital tumours. 5th ed. World Health Organization; 2020. WHO Classification of Tumours. Vol 4

2. Strom SP. Current practices and guidelines for clinical next-generation sequencing oncology testing. Cancer Biol Med. 2016;13(1):3-11. doi:10.28092/j.issn.2095-3941.2016.0004

3. Spurr L, Li M, Alomran N, et al. Systematic pan-cancer analysis of somatic allele frequency. Sci Rep. 2018;8(1):7735. Published 2018 May 16. doi:10.1038/s41598-018-25462-0

Marcus L, Lemery SJ, Keegan P, Pazdur R: FDA Approval Summary: Pembrolizumab for the treatment of microsatellite instability-high solid tumors. Clin Cancer Res. 2019;25(13):3753-3758

4. Cancer Genome Atlas Research Network; Kandoth C, Schultz N, et al: Integrated genomic characterization of endometrial carcinoma [published correction appears in Nature. 2013 Aug 8;500(7461):242]. Nature. 2013;497(7447):67-73. doi:10.1038/nature12113

5. Cancer Genome Atlas Research Network. Integrated genomic analyses of ovarian carcinoma [published correction appears in Nature. 2012 Oct 11;490(7419):298]. Nature. 2011;474(7353):609-615

Next-generation sequencing is performed to determine microsatellite instability (MSI) status and evaluate the presence of a mutation in most coding regions of the AKT1, APC, ARID1A, ARID1B, ATM, ATR, ATRX, BAP1, BARD1, BRAF, BRCA1, BRCA2, BRIP1, CDC42, CDK12, CDKN2A, CTNNB1, DICER1, EIF1AX, EPCAM, , ERBB2, FBXW7, FOXL2, GNAS, KRAS, L1CAM, MED12, MLH1, MSH2, MSH6, NBN, NF2, NRAS, PALB2, PIK3CA, PIK3R1, PMS2, POLE, PPP2R1A, PTEN, RAD51C, RAD51D, RB1, SMARCA2, SMARCA4, SMARCB1, STK11, TP53, and TRAF7 genes. See Targeted Genes and Methodology Details for MayoComplete Gynecological Panel for details regarding the targeted gene regions identified by this test.(Unpublished Mayo method)

A pathology review and macro dissection to enrich for tumor cells is performed prior to slide scraping.

Monday through Friday

• Authorized users can sign in to Test Prices for detailed fee information.
• Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
• Prospective clients should contact their account representative. For assistance, contact Customer Service.

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

88381-Microdissection, manual

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