Test Catalog

Test Id : PPAP

Order This Test

Parental Sample Prep for Prenatal Microarray Testing, Blood

Download
Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Preparing parental blood specimen for possible confirmation testing if an abnormality is detected on the prenatal array sample

 

DNA extraction of the maternal blood specimen used for maternal cell contamination testing

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.

 

When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.

Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.

Method Name
A short description of the method used to perform the test

DNA Extraction/Cell Culture

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Parental Prenatal Array Prep Test

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.

 

When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.

Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.

Specimen Type
Describes the specimen type validated for testing

Whole blood

Additional Testing Requirements

This test must be ordered in conjunction with CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue. Each parent should have a specimen collected for this test ID using a different order number than the fetal specimen collected for the CMAP, CMAPC, or CMAMT order.

Necessary Information

1. The reason for testing is required.

2. Include the name listed on the prenatal specimen that was submitted for CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

This test requires 2 blood specimens: 1 sodium heparin and 1 EDTA

 

Specimen Type: Whole blood

Container/Tube: Green top (sodium heparin) and lavender top (EDTA)

Specimen Volume: 3 mL EDTA and 4 mL sodium heparin

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimens in original tubes. Do not aliquot.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

2 mL EDTA and 2 mL sodium heparin whole blood

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred)
Refrigerated

Useful For
Suggests clinical disorders or settings where the test may be helpful

Preparing parental blood specimen for possible confirmation testing if an abnormality is detected on the prenatal array sample

 

DNA extraction of the maternal blood specimen used for maternal cell contamination testing

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test contains no charge and serves as a way to correlate proband and parental specimens. If additional testing is warranted, the appropriate tests will be added.

 

When CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling; CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth, Varies; or CMAMT / Chromosomal Microarray, Autopsy/Products of Conception/Stillbirth, Tissue is ordered, parental blood specimens are collected and prepared for confirmation studies should an abnormality be detected on the prenatal sample. If an abnormality is detected on the fetal specimen, the most appropriate testing (microarray, FISH, or chromosome analysis) to aid in the interpretation of the prenatal result can be performed on the parental specimens. A paternal blood specimen is requested but not required.

Maternal cell contamination testing will be performed on the maternal blood and prenatal specimens to rule out the presence of maternal cells in the fetal sample.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

In order to interpret equivocal array results on a prenatal sample (amniotic fluid or chorionic villus), parental studies are performed to determine if the abnormality detected on the prenatal array is inherited or de novo.

 

Maternal cell contamination testing is performed on the maternal blood and prenatal specimen to detect the presence of maternal cells in the fetal sample.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

An interpretive report will be provided.

Interpretation
Provides information to assist in interpretation of the test results

No interpretation will be provided. This test is for specimen processing only.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Method Description
Describes how the test is performed and provides a method-specific reference

The heparinized sample will be cultured following standard laboratory protocols and a fixed-cell pellet will be prepared. DNA will be extracted from the EDTA sample and used to perform maternal cell contamination testing on the maternal sample. The fixed cell pellet and DNA will be stored in the laboratory for possible confirmation testing if an abnormality is detected on the prenatal array sample.(Unpublished Mayo method)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Varies

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

4 weeks

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

Not Applicable

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

This test ID contains no charge and serves as a way to correlate proband parental specimens. If additional testing is warranted, the appropriate tests will be added.

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
PPAP Parental Prenatal Array Prep Test In Process
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
55079 Result Summary 50397-9
52978 Interpretation 69965-2
52979 Reason For Referral 42349-1
53337 Specimen 31208-2
53338 Source 31208-2
53339 Method 85069-3

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports