Test Catalog

Test Id : FBL

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Fungal Culture, Blood

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis and treatment of the etiologic agents of fungemia

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
D2F D2 Fungal Sequencing Identification No, (Bill Only) No
FUNA Fungal Ident Panel A No, (Bill Only) No
FUNB Fungal Ident Panel B No, (Bill Only) No
LCCI Ident Rapid PCR Coccidioides No, (Bill Only) No
LCHB Id, Histoplasma/Blastomyces PCR No, (Bill Only) No
RMALF Id MALDI-TOF Mass Spec Fungi No, (Bill Only) No
RMALY Id MALDI-TOF Mass Spec Yeast No, (Bill Only) No
LCCA Id, Candida auris Rapid PCR No, (Bill Only) No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, reflex testing may be performed at an additional charge.

Method Name
A short description of the method used to perform the test

Conventional Broth Culture/Macroscopic/Microscopic/D2 rDNA Gene Sequencing/Real-Time Polymerase Chain Reaction (PCR)/Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS)

 

Dimorphic Pathogen Identification Confirmation: D2 rDNA Gene Sequencing/PCR/MALDI-TOF MS

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Fungal Culture, Blood

Aliases
Lists additional common names for a test, as an aid in searching

Culture, Fungus

Culture, Yeast

Fungus Culture

Mold

Mycology Culture

Yeast Culture

50022-FBL

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, reflex testing may be performed at an additional charge.

Specimen Type
Describes the specimen type validated for testing

Whole blood

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
Q00M0019 Specimen Source

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube:

Preferred: Green top (sodium or lithium heparin)

Acceptable: SPS

Specimen Volume: 4 mL

Pediatric Volume: 3 mL

Collection Instructions:

1. Send whole blood specimen in original tube. Do not aliquot.

2. SPS tubes are acceptable, but not preferred.

Note: SPS tubes must be clearly labeled as SPS. If label is obscured, sample may be cancelled, as ACD (also yellow top) is not an acceptable tube type.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.

Adults: 3 mL

Pediatrics: 1 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Blood culture bottles (eg, BACTEC MycoF Lytic)
Isolator
Clotted 		Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred) 7 days
Refrigerated 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis and treatment of the etiologic agents of fungemia

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, reflex testing may be performed at an additional charge.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Due to the high mortality rate from fungemia, the expeditious detection and identification of fungi from the patient's blood can have great diagnostic prognostic importance. Risk factors for fungemia include, but are not limited to, extremes of age, immunosuppression, and those individuals with burns or indwelling intravascular devices.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative

If positive, notification is made as soon as the positive culture is detected or identified.

Interpretation
Provides information to assist in interpretation of the test results

Positive cultures of yeast and filamentous fungi are reported with the organism identification.

 

Positive cultures are usually an indication of infection and are reported as soon as detected. Correlation of culture results and the clinical situation is required for optimal patient management. A final negative report is issued after 42 days of incubation.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Procop GW, Church DL, Hall GS, et al. Mycology. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Walters Kluwer; 2017:1322-1416

2. Zheng S, Ng TY, Li H, Tan AL, Tan TT, Tan BH. A dedicated fungal culture medium is useful in the diagnosis of fungemia: a retrospective cross-sectional study. PLoS One. 2016;11(10):e0164668. doi:10.1371/journal.pone.0164668

3. Magallon A, Basmaciyan L, Chapuis A, et al. Evaluation of the relevance of use of the BD-BACTEC MycosisIC/F, BD-BACTEC PlusAerobic/F, BD-BACTEC Lytic/10 anaerobic/F and BD-BACTEC PedsPlus/F culture bottle system for fungemia detection: A 4-year retrospective study at the Dijon university hospital, France. J Mycol Med. 2022;32(4):101295. doi:10.1016/j.mycmed.2022.101295

Method Description
Describes how the test is performed and provides a method-specific reference

Blood is inoculated into a MycoF Lytic culture bottle (Becton Dickinson) bottle and continuously monitored on a blood culture instrument.

 

Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, laboratory-developed real-time polymerase chain reaction assays and/or D2 rDNA gene sequencing, as applicable.(Babady NE, Buckwalter SP, Hall L, Le Febre KM, Binnicker MJ, Wengenack NL. Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR. J Clin Microbiol. 2011;49[9]:3204-3208; Binnicker MJ, Buckwalter SP, Eisberner JJ, et al. Detection of Coccidioides species in clinical specimens by real-time PCR. J Clin Microbiol. 2007;45[1]:173-178; Dhiman N, Hall L, Wohlfiel SL, Buckwalter SP, Wengenack NL. Performance and cost analysis of matrix-assisted laser desorption ionization time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol. 2011:49[4];1614-1616; Hall L, Wohlfiel SL, Roberts GD. Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. J Clin Microbiol. 2004;42[2]:622-626; Theel ES, Schmidt BH, Hall L, et al. Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2012;50[9]:3093-3095; Theel ES, Hall L, Mandrekar J, Wengenack NL. Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011;49[12]:4067-4071)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

42 to 45 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87103-Blood

87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107-Fungal identification panel A (if appropriate)

87107-Fungal identification panel B (if appropriate)

87150-Identification rapid PCR coccidioides (if appropriate)

87150 x 2- Identification Histoplasma/Blastomyces, PCR (if appropriate)

87153-D2 fungal sequencing identification (if appropriate)

87150- Id, Candida auris Rapid PCR (if appropriate)

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
FBL Fungal Culture, Blood 601-5
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
FBL Fungal Culture, Blood In Process

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports

Test Update Resources

Change Type Effective Date
Test Status - Test Resumed 2024-08-20
Test Status - Test Down 2024-06-25
Test Changes - Specimen Information 2023-08-08